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. 2021 Aug 14;52:108. doi: 10.1186/s13567-021-00976-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Cellular composition after transfer of equine enteroids to 2D monolayers. Enteroids could be dissociated to single cells and re-established on pre-coated plates or transwell inserts. A Graphical illustration of enteroid and monolayer cultures. B Monolayer after three days of culture on a flat-bottomed plate. Contrast stained by hematoxylin (Photo: Nikon D40x). C Transepithelial electrical resistance in transwell cultures monitored over 15 days (mean value ± SD, two technical replicates). D Expression of the cell lineage markers SOX9, LYZ, PCNA, EPCAM, CGA, MUC2 and DCLK1 in enteroids or in 2D monolayers cultured on plates or transwell inserts. The gene expression was normalized to the geometric mean for the reference genes (HPRT, SDHA and GAPDH) and is presented as 2^−ΔCt. The results are given as mean values ± SD for cultures established from each of the three experimental horses (n = 3). P < 0.05 = * and P < 0.001 = ***.