Fig. 2.

Immunization with HMW1+HMW2 reduces nasopharyngeal colonization density. Mice were immunized with purified HMW1₁₂+HMW2₁₂ (A) or HMW1₅+HMW2₅ (B) and subsequently challenged with NTHi strain 12, strain 5, strain 12hmw1hmw2, or strain NT127. Inoculums were ∼10⁸ CFU. The density of colonization was represented as log(recovered CFU/g nasal tissue). Data represent means ± SD of five animals per group. Significance was determined using the Kruskall–Wallis test. If a significant variance was identified, the Mann–Whitney U test was used to compare individual groups to adjuvant controls. n.s., not significant. *P < 0.05.