Fig. 5.

The chaperone XL-PPI networks. (A) The proteins identified with direct interactions with at least one of the chaperones were illustrated as individual nodes. The chaperones were grouped by seven major families shown as octagons. Chaperone-interacting proteins were color coded based on which chaperones they interacted with. Blending of colors was used when proteins interacted with multiple chaperones. (B) Hsp70-associated CORUM protein complex network defined by Hsp70 XL PPIs, illustrating connectivity among protein complexes. Complexes at the innermost ring only had direct interactions with Hsp70, while complexes at outer rings interacted with both Hsp70 and its associated complexes. (C) The interaction network of the three circled complexes from B arranged into subunits that compose these complexes, that is, sororin-cohesin, histones, and spliceosome C complexes. (D) 2D XL maps of the interactions seen between the subunits shown in C. Domains of interest were colored according to the scheme shown.