Fig. 2.

Metabolic distribution of U-¹³C isotopic-labeled OA and glycerol reveals an altered metabolic flux resulting in a preferential assimilation of OA at pH 5.0. The percentage of isotopically labeled metabolite pools were determined after 8-h incubation with 1.2 mM [U-¹³C]Gly, 200 μM [U-¹³C]OA, [U-¹³C]Gly + OA, and [U-¹³C]OA + Gly at pH 7.0 and pH 5.0. Each bar represents the percentage of the total pool that is ¹³C labeled. Blue bars indicate that the ¹³C labeling is derived from ¹³C-glycerol (Gly*), and red bars indicated that the labeling is derived from ¹³C- OA*. Data are the means ± SD of triplicates and are representative of two independent experiments. Statistical significance between the percentage of ¹³C-labeled pools at pH 7 and pH 5 for each metabolite was determined using unpaired t test. *P < 0.05; **P <0.005; ***P < 0.0005; ****P < 0.0001; ns = not significant. Gly = glycerol, OA = oleic acid, α-KG = α-ketoglutarate, C = carbon.