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. 2021 Aug 5;118(32):e2106311118. doi: 10.1073/pnas.2106311118

Fig. 2.

Fig. 2.

Impact of Gata3 +674/762 deletion on homeostatic ILC2 function. (A) Representative histogram and summary bar graph of GATA3 median fluorescent intensity (MFI) in lung ILC2s and summary bar graph for CD4SP thymocytes. White histogram denotes lung ILC2 GATA3 stain blocked with unlabeled antibody (cold competitor). (B) Summary bar graph of normalized IL-33Rα MFI on lung ILC2s. (C) Summary data of lung eosinophil cell numbers. (D) Representative flow cytometry plots and summary data for frequency of IL-5 and IL-13 cytokine production from lung ILC2s (pregated on CD45.2+CD19−CD11c−CD3ε−TCRβ−IL-7Rα+CD90.2+CD25+IL-33Rα+) stimulated in vitro with PMA and ionomycin for 4 h. (E) Schematic for establishing congenically marked mixed bone marrow chimeric mice. (F) Gata3 +674/762Δ/Δ:WT reconstitution ratio for the indicated populations in mixed bone marrow chimeric mice. Dots represent individual mice; n ranging from 6 to 10 in different groups pooled from multiple independent experiments; data are presented as mean ± SEM. Statistical comparison was performed via unpaired t test, multiple unpaired t test, or one-way ANOVA. **P < 0.01; ****P < 0.0001.