Figure 3.

Validation experiments on cell cycle regulator candidate genes. A, Heatmap (row-scaled) visualizing the expression of candidate genes in the pericycle cell clusters from the single-cell library. Scale bar represents the z-score of the normalized expression values. B, Confocal microscopy images of candidate genes’ transcriptional reporters in early-stage LRP (left) and bright-field image of the corresponding primordia (right, Arrow indicate LRP). C, Lateral root density of J0121^Col≫dCas9R transgenic lines of candidate genes. D, Proportion of total root length contributed by lateral roots of J0121^Col≫dCas9R transgenic lines of candidate genes. For C and D, significance was determined by pairwise comparison with empty vector control and ANOVA (ns: P > 0.05 | ^*P ≤ 0.05 | ^**P ≤ 0.01 | ^***P ≤ 0.001 | ^****P ≤ 0.0001; Supplemental File S1). For each candidate gene, multiple independent transgenic lines have been analyzed; each colored line in the graph represents an individual line. All seedlings were imaged and analyzed at 10 dpg.