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. 2021 May 2;33(7):2320–2339. doi: 10.1093/plcell/koab120

Figure 1.

Figure 1

Expression analysis of SlPIF4. A, Expression profile of SlPIF4 in tomato leaves and flowers. “Micro-Tom” seedlings were grown under long-day conditions (16-h/8-h light/dark photoperiod) for 6 weeks, and RNA samples were prepared from the third fully expanded leaf and flower buds at the binucleate stage at a 4-h interval. Three individual plants were used for sampling at each time point. 0 h: 6 a.m. The dark periods are indicated by gray shading. Each value is the mean ± sd (n = three biological replicates). Expression levels shown are relative to that at time point 0, which was set at 1. B and C, Expression analysis of SlPIF4 in various tissues using qRT-PCR (B) and in WT anthers by in situ hybridization (C). Each value indicates the mean ± sd (n = three technical replicates, each organ was selected from at least five individual WT plants). Expression levels shown in (B) are relative to that in roots, which was set at 1. R, roots; S, stems; L, leaves; GF, green fruits; I, early meiosis stage; II, tetrad stage; III, early uninucleate stage; IV, late uninucleate stage; V, binucleate stage; VI, mature pollen stage. MMC, microspore mother cell; Msp, microspore; T, tapetum; Td, tetrads. Bars = 100 µm.