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. 2021 May 2;33(7):2320–2339. doi: 10.1093/plcell/koab120

Figure 3.

Figure 3

Knocking out SlPIF4 reduces the sensitivity of the tapetum to moderately low-temperature conditions. TUNEL assays of tapetal PCD in WT and slpif4 anthers under NT and MLT conditions at the tetrad, early, middle and late uninucleate, and late binucleate stages. The magenta signal is propidium iodide staining, and the green fluorescence indicates TUNEL positive signal (white arrows). At least seven anthers in each stage were sampled for TUNEL assays after 6-week-old WT and slpif4 plants were exposed to MLT (15°C/15°C, day/night) and NT (25°C/20°C, day/night) for 6 d. Bars = 50 µm.