Relationship between endosperm and seed area and effect of seed area on germination. A, Seed area in each cross (M. guttatus—2x × 2x [red]; 2x × 4x [orange]; M. luteus—4x × 4x [blue]; 4x × 2x [green]) is represented in violin plots. Dark red diamonds display group means. Letters represent Tukey–Kramer results following an ANOVA. Groups with different letters are statistically different in area. B, Regression between endosperm area and area of the entire seed. The r2 value is provided. C, Area of seeds that failed to germinate compared with successfully germinated seeds among all crosses. 4x × 2x seeds are not included to avoid biasing the comparison since none germinated, and they were the smallest of the four crosses. Dark red diamonds represent group means and the asterisk indicates they are statistically different (P < 0.05, Welch’s t test).