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. 2021 Aug 10;14:4415–4426. doi: 10.2147/OTT.S297336

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© 2021 Zhang et al.

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Knockdown of TRG-AS1 inhibited tumor growth in vivo. (A) The expression of TRG-AS1 in mice tumor tissues infected with Lv-sh-control or Lv-sh-TRG-AS1 was detected by RT-qPCR. (B) The expression of miR-224-5p in mice tumor tissues infected with Lv-sh-control or Lv-sh-TRG-AS1 was detected by RT-qPCR. (C) Tumor volume of Lv-sh-control or Lv-sh-TRG-AS1 mice tumor was measured. (D) Ki-67 immunohistochemistry (B), Western blots analysis of SMAD4 expression (E) and SMAD4 immunohistochemistry (F) in mice of Lv-sh-control group or Lv-sh-TRG-AS1 group. All experiments were repeated 3 times. *p < 0.05.