Figure 2.

Angiogenic pathways may be upregulated by ALN treatment. (A) Secretion of VEGF in MG-63 bone cells treated with ALN. Effect of ALN treatment on the secreted VEGF levels into conditioned medium by MG-63 cells. Values (mean and standard deviation (SD)) are expressed as fold-changes compared to untreated cells (Ctrl, control). (B) Proliferation of HUVEC in the collected media of MG-63 cells. **p < 0.001, compared to control (Student's t-test). (C-D) No apoptosis was observed within the treatment period of 6 h. (C). Cell viability of MG-63 cells. MTT assay revealed no viability changes by ALN treatment. (D) Crystal violet staining assay showed no cell mass changes in response to varying concentration of ALN for 6 days. Experiments were done 6 times. Representative images were shown.