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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Trends Cell Biol. 2021 Mar 22;31(9):760–773. doi: 10.1016/j.tcb.2021.03.005

Figure 3. The topology of replicated genome.

Figure 3.

(A) By the specific labelling of Watson (W) or Crick(C) strands during DNA replication in S phase, sister-chromatid-sensitive Hi-C approach (scsHi-C) allowed the resolution of chromatin contacts between sister chromatids at S and G2 stages of the cell cycle. (B) Two classes of CTCF loops emerged. Paired CTCF loops, with high level of trans interactions between sister chromatids that correspond to polycomb-repressed heterochromatin (PRH). In contrast, unpaired CTCF loops show abundant cis interactions and poor inter-chromatid connection that correlate with active transcription. In both cases, Sororin and Esco2 stabilizes replicative cohesin showing enrichment at CTCF loop boundaries.