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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Trends Parasitol. 2021 Jun 22;37(9):803–814. doi: 10.1016/j.pt.2021.05.013

Figure I (in Box 1). Plate sorted mixtures result in pure genome sequences.

Figure I (in Box 1).

(A) Parasites with known genotypes (A and C) are mixed, analyzed and sorted by FACS. After whole genome amplification, library preparation and sequencing, the bioinformatic analysis show unique genotype for each sorted single cell showing the absence of contamination between cells. (B) Contamination between cells can be detected by the genome size analysis. Mapping the genotypes will enable distinguishing a contamination from different genotypes (C) A single locus of the mitochondrial genome is shown for 3 3D7 and 3 Hb3 cells. All the reads at each locus match either 3D7 or Hb3 with no cross-contamination. This was consistent across all reliably called loci. In the lower panel an example of a mixed cell is shown.