(A) hES-MP cells express mesenchymal surface markers CD73 and CD105. EWSR1(ex7)-CREB1(ex7) and EWSR1(ex7)-ATF1(ex5) refer to cells carrying the translocation prior to Cre expression.
(B) Time course in days of EWSR1-CREB1 fusion expression in hES-MP cells after Cre expression. The AFH core gene signature is detected in hES-MP cells expressing the EWSR1(ex7)-CREB1(ex7) fusion over time. Histogram represents the fold increase compared to wild type cells (-Cre condition) and the error bars the standard deviation from the mean of 4 independent experiments. Statistical significance is calculated with a paired t-test comparing +Cre with the corresponding -Cre condition. *p<0.05, **p<0.01; when not indicated the difference is considered not statistically significant.
(C) Same as panel B for the EWSR1(ex7)-ATF1(ex5) fusion. qRT-PCR in hES-MP cells expressing the EWSR1(ex7)-ATF1(ex5) at different time points. Statistical analysis as in panel B, n=5. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.