Table 3.
Percentage of correct and incorrect final molecular interpretations in wet and paper-based cases
| Final molecular interpretation in EQA schemes 2014–2018 | IG analysis | TR analysis | ||||
|---|---|---|---|---|---|---|
| N=1380 | Paper | Wet | N=1345 | Paper | Wet | |
| Consensus: clonality detected | 1047 (75.9) | 674 (64.4) | 373 (35.6) | 913 (67.9) | 551 (60.4) | 362 (39.6) |
| Clonality detected | 1021 (97.5) | 666 (65.2) | 355 (34.8) | 893 (97.8) | 533 (59.7) | 360 (40.3) |
| Polyclonality detected (no clonality detected) | 19 (1.8) | 6 (31.6) | 13 (68.4) | 9 (1.0) | 8 (88.9) | 1 (11.1) |
| No rearrangement in IG/TR targets detected | 5 (0.5) | 1 (20.0) | 4 (80.0) | 1 (0.1) | 1 (100.0) | 0 (0.0) |
| No clonality detected, suggestive of low template amount | 1 (0.1) | 0 (0.0) | 1 (100.0) | 0 (0.0) | / | / |
| Oligoclonality/multiple clones detected | 1 (0.1) | 1 (100.0) | 0 (0.0) | 7 (0.8) | 6 (85.7) | 1 (14.3) |
| Not evaluable | 0 (0.0) | / | / | 3 (0.3) | 3 (100.0) | 0 (0.0) |
| Consensus: oligoclonality/multiple clones detected | 57 (4.1) | 57 (100.0) | 0 (0.0) | 107 (7.9) | 107 (100.0) | 0 (0.0) |
| Oligoclonality/multiple clones detected | 7 (12.3) | 7 (100.0) | 0 (0.0) | 27 (25.2) | 27 (100.0) | 0 (0.0) |
| Clonality detected | 45 (78.9) | 45 (100.0) | 0 (0.0) | 76 (71.1) | 76 (100.0) | 0 (0.0) |
| Polyclonality detected (no clonality detected) | 5 (8.8) | 5 (100.0) | 0 (0.0) | 3 (2.8) | 3 (100.0) | 0 (0.0) |
| Not evaluable | 0 (0.0) | / | / | 1 (0.9) | 1 (100.0) | 0 (0.0) |
| Consensus: polyclonality detected (no clonality detected) | 276 (20.0) | 116 (42.0) | 160 (68.0) | 269 (20.0) | 107 (39.8) | 162 (60.2) |
| Polyclonality detected (no clonality detected) | 265 (96.1) | 112 (42.3) | 153 (57.7) | 251 (93.3) | 105 (41.8) | 146 (58.1) |
| No rearrangement in IG/TR targets detected | 7 (2.5) | 4 (57.1) | 3 (42.9) | 5 (1.9) | 0 (0.0) | 5 (100.0) |
| Clonality detected | 2 (0.7) | 0 (0.0) | 2 (100.0) | 13 (4.8) | 2 (15.4) | 11 (84.6) |
| No clonality detected, suggestive of low template amount | 2 (0.7) | 0 (0.0) | 2 (100.0) | 0 (0.0) | / | / |
| No consensus: educational case | 0 (0.0) | / | / | 56 (4.2) | 56 (100.0) | 0 (0.0) |
| Clonality detected | 0 (0.0) | / | / | 31 (55.4) | 31 (100.0) | 0 (0.0) |
| Polyclonality detected (no clonality detected) | 0 (0.0) | / | / | 22 (39.3) | 22 (100.0) | 0 (0.0) |
| No clonality detected, suggestive of low template amount | 0 (0.0) | / | / | 2 (3.6) | 2 (100.0) | 0 (0.0) |
| Not evaluable | 0 (0.0) | / | / | 1 (1.8) | 1 (100.0) | 0 (0.0) |
EQA, external quality assessment; IG, immunoglobulin gene; N, number of samples tested; TR, T-cell receptor gene