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. 2021 Aug 14;7:215. doi: 10.1038/s41420-021-00595-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Online bioinformatics tool (https://circinteractome.nia.nih.gov/, http://rbpdb.ccbr.utoronto.ca/) found that circARHGAP12 might interact with the RNA binding protein (RBP) IGF2BP1 and IGF2BP2. B RBP immunoprecipitation (RIP) assay following qPCR demonstrated the enrichment of circARHGAP12 in the anti-IGF2BP2 or IgG antibody group (CaSki, SiHa). C Fluorescence in situ hybridization (FISH) assay illustrated the distribution of circARHGAP12 and IGF2BP2 in cervical cancer (CaSki) cells. CircARHGAP12 was labeled with the FAM (green) and IGF2BP2 was labeled with the cy3 (red). D, E Biotin-labeled RNA pull-down was performed using circARHGAP12 probe and control probe. The enrichment of circARHGAP12 was measured using RT-qPCR and the level of IGF2BP2 protein was detected using western blot assay. Experiments were performed in triplicate. P values were calculated by Student’s t-test. **p < 0.01.