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. 2021 Aug 14;11(8):144. doi: 10.1038/s41408-021-00536-x

Fig. 2. IL-10 CAR-T cells exhibited antigen-specific cytotoxicity against AML cell lines.

Fig. 2

a Schematic diagram of the IL-10 CAR. b Representative flow cytometry analysis showing the expression of GFP and IL-10 on VEC-T cells or IL-10 CAR-T cells. c Quantification and statistical analysis of the data in (b). d The expression of IL-10RA (upper panel) or IL-10RB (lower panel) in five leukemia cell lines (MV4-11, Kasumi-1, U937, Thp-1, and Molm-13; iso, isotype control). e Quantification and statistical analysis of the mean fluorescence intensity (MFI) of (d) (n = 3). f Quantification and statistical analysis of CD69 expression in VEC-T or IL-10 CAR-T cells (GFP+) upon leukemia cells stimulation for 6 h (n = 3; **p < 0.01; ***p < 0.001). g Quantification and statistical analysis of CD25 expression in VEC-T or IL-10 CAR-T cells (GFP+) after 48 h cocultured with leukemia cells (n = 3; *p < 0.05; **p < 0.01; ***p < 0.001). h Quantification and statistical analysis of CD107a released by VEC-T or IL-10 CAR-T cells (GFP+) after 6 h cocultured with leukemia cells (n = 3; *p < 0.05; **p < 0.01; ***p < 0.001). i Quantification and statistical analysis of Granzyme B (GZMB) released by VEC-T or IL-10 CAR-T cells (GFP+) after 6 h cocultured with leukemia cells (n = 3; *p < 0.05; **p < 0.01). j Effect cells and target cells were cocultured for 48 h at the indicated E:T ratio (1:4, 1:2, 1:1, 2:1, 4:1). Flow cytometry analysis of the percentage of CD3− cells, which represented for the residue of leukemia cell (n = 3, two-way ANOVA; n.s. no significant; **p < 0.01; ***p < 0.001; ****p < 0.0001). k ELISA data showing the release of INF-γ, TNF-α, IL-2, and IL-6 by VEC-T or IL-10 CAR-T cells after coculture with target cells at the E:T ratio of 1:1 for 48 h (n = 3; n.s. no significant; *p < 0.05; **p < 0.01).