Fig. 5. IL-10 CAR-T cells have few off-target effects on the hematopoietic system.

Representative flow cytometry analysis showed the proportion of CD34⁺ UBC cells after sorted by magnetic beads. b Representative flow cytometry analysis showed the expression of IL-10RA (left panel) and IL-10RB (right panel) on CD34⁺ UCB cells. c Quantification and statistical analysis of the data in (b) (n = 6). d Quantification and statistical analysis of the expression of IL-10RA and IL-10RB in different subgroups of cells (progenitor cell, CD34⁺ CD38⁺; granulocyte and monocyte precursor cell; CD34⁺ CD33⁺; hematopoietic stem cell, CD34⁺ CD38⁻). e Totally, 5 × 10⁴ CD34⁺ UCB cells which were untreated, or cocultured with 5 × 10⁴ VEC-T or IL-10 CAR-T cells for 24 h. Quantification and statistical analysis of the proportion of CD3⁺ and CD34⁺ cells which were detected by flow cytometry (n = 3; n.s. no significant). f Representative images showed the colony formation of CD34⁺ UCB cells which were untreated or cocultured with 5 × 10⁴ VEC-T or IL-10 CAR-T cells. h Quantification and statistical analysis of the data in (f) (n = 3; two-way ANOVA; n.s. no significant).