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. 2021 Aug 14;11(8):144. doi: 10.1038/s41408-021-00536-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Representative flow cytometry analysis of the expression of IL-10RA (left panel) and IL-10RB (right panel) on T cells. b Quantification and statistical analysis of the MFI in (a) (n = 9). c Schematic diagram of the VEC-CS and IL-10 CAR constructs. d The proliferation of VEC-CS T or IL-10 CAR-T cells cultured for 21 days (n = 3). e Quantification of the apoptosis of VEC-CS T or IL-10 CAR-T cells at day 7 and day 14 (n = 3; two-way ANOWA; n.s. no significant). f Quantification and statistical analysis of the proportion of CD4⁺ or CD8⁺ T cells at day 14 (n = 9; *p < 0.05). g Quantification and statistical analysis of the proportion of CD4⁺ CD25⁺ FOXP3⁺ Tregs at day 7 (n = 5; **p < 0.01). h Representative flow cytometry analysis showing the expression of T-bet (left panel) and GATA-3 (right panel) on T cells. i Quantification and statistical analysis of the MFI in (h) (n = 3; n.s. no significant). j Quantification and statistical analysis of the proportion of T_SCM (CD45RA⁺ CCR7⁺), T_CM (CD45RA⁺ CCR7⁻), T_EM (CD45RA⁻ CCR7⁺), and T_EMRA (CD45RA⁺ CCR7⁻) subgroups in VEC-CS T or IL-10 CAR-T cells. CD4⁺ T cell analysis was shown in the left panel, and CD8⁺ T cell analysis was shown in the right panel (n = 9; two-way ANOWA; n.s. no significant; *p < 0.05).