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. 2021 Aug 14;11(8):144. doi: 10.1038/s41408-021-00536-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Schematic diagram of the murine IL-10 CAR (structures containing murine IL-10 followed by the murine CD8α hinge and transmembrane domain, intracellular CD28 or 4-1BB costimulatory domain, and intracellular CD3ζ signaling domain. b Representative flow cytometry analysis showing transduction efficiency of mVEC-T, mIL-10-CD28 CAR-T, or mIL10-4-1BB CAR-T cells. c Schematic diagram of the experimental regimen. d The gating strategy of flow cytometric analysis showing the method to distinguish immunosuppressive cells in BM microenvironment (M-MDSC, monocytic-myeloid derived suppressor cells; PMN-MDSC, polymorphonuclear-myeloid derived suppressor cells; TAM, tumor associated macrophage). e Quantification and statistical analysis of the proportion of M-MDSC, PMN-MDSC, TAM, and Treg (CD45⁺ CD11b⁺ Ly6C⁺ Ly6G⁻, M-MDSC; CD45⁺ CD11b⁺ Ly6C^low Ly6G⁺, PMN-MDSC; CD45⁺ CD11b⁺ F4/80^high, TAM; CD4⁺ CD25⁺ CD127⁻, Treg; n.s. no significant; **p < 0.01).