Table 4.

Summary of minimum inhibitory concentration^a (µg/mL) and minimum bactericidal concentration (MBC)^b (µg/mL) against Escherichia coli O157:H7 injured by antimicrobials using three different methods

Antimicrobials	Broth dilution method		Agar recovery method
	Antimicrobial fixed broth dilution method	Metabolic inhibitor fixed broth dilution method	Metabolic inhibitor fixed agar recovery method
Ethanol	Rifampicin D-cycloserine Streptomycin	Rifampicin Streptomycin	Nalidixic acid
EDTA	Chloramphenicol Nalidixic acid Rifampicinc Penicillin D-cycloserine Streptomycin Cerulenin	Chloramphenicol Nalidixic acid Rifampicin Penicillin D-cycloserine Streptomycin	NDd
Polymyxin B	Rifampicin D-cycloserine	ND	Nalidixic acid Rifampicin
Thymol	Chloramphenicol Nalidixic acid Rifampicin Penicillin D-cycloserine Streptomycin	Nalidixic acid	Chloramphenicol Nalidixic acid Rifampicin Penicillin G D-cycloserine Streptomycin Cerulenin
Acetic acid	Rifampicin D-cycloserine	D-cycloserine	Rifampicin
Citrus fruit extract	Chloramphenicol Nalidixic acid Rifampicin D-cycloserine	ND	Nalidixic acid Cerulenin

^aMIC was determined as the minimum concentration that resulted in < 0.4 an optical density at 595 nm (non-bold letter)

^bMBC was determined as the minimum concentration that resulted in < 0.1 an optical density at 595 nm (bold letter)

^cND not determined