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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: J Neurochem. 2021 Jan 18;157(3):752–763. doi: 10.1111/jnc.15281

Figure 4. FUS regulated the protein and mRNA levels of proteins involved in early phases of the autophagy pathway.

Figure 4.

N2A and FUS KO cells were cultured and immunoblotting was performed using the indicated antibodies. (B-C) Quantification of (A) from three independent t-test was performed for individual comparisons against N2A WT cells. *, p ≤0.05; N.S., not significant. (D) N2A and FUS KO cells were cultured and immunoblotting was performed using the indicated antibodies. (E) Quantification of (D) from three independent t-test was performed for individual comparisons against WT N2A WT cells. *, p ≤0.05; N.S., not significant. (F-G). N2A and FUS KO cells were cultured and harvested for RNA isolation, followed by reverse transcription and quantitative PCR using TaqMan probes for the indicated genes. Actin probe was used to normalize gene expression. Results from three (F) or four (G) independent experiments are shown. Student’s t-test was performed for individual comparisons against N2A WT cells. *, p ≤0.05; N.S., not significant. All quantification results are shown in scatter plots with the bar representing the median.