5-Aza inhibited the proliferation of prostate cancer cells. A) DU145, 22RV1, LNCaP and RWPE-1 cells were treated with 5-Aza at 0.5-8 μM for 96 h. The cell viability was detected using a WST-1 assay. The data from each processing point represent the means ± SD of triplicate wells. Abbreviations: 5-Aza, 5-Aza-2′-deoxycytidine; SD, standard deviation. B) DU145, 22RV1, LNCaP and RWPE-1 cells were treated with 5-Aza at 2 µM, 4 µM and 8 µM for 96 h. The cell viability was detected using a trypan blue staining assay. The light microscopy was used to investigate the cell phenotype. Scal bar: 20µm. Abbreviations: 5-Aza, 5-Aza-2′-deoxycytidine.