Figure 5.

Stromal fibroblasts are activated in the tumor microenvironment of CRC patients. (A) Immunofluorescence staining for Vimentin (red), α-SMA (green) and FAP (green) in the isolated primary fibroblasts (400×). NF, normal fibroblasts; PF, pericancerous fibroblasts; CAF, cancer associated fibroblasts. Nuclei (blue) were labelled by DAPI. (B) Flow cytometry analysis of the expression of Vimentin in the three types of isolated primary fibroblasts. All experiments were performed independently at least three times