The expression of CLDN6 in hHCC tissues and cells. A, TCGA database was used to analyze CLDN6 expression in hHCC patients. B, immunohistochemical staining technology was used to determine the expression of CLDN6 in hHCC tissues. C, RT-PCR and western blotting analysis was performed to determine the expression of CLDN6 in hHCC cells HepG2 and Hep3B. D, immunocytochemistry was utilized to detect the expression and location of CLDN6 in hHCC cells HepG2 and Hep3B, the black arrows point out typical cells that express CLDN6. (*p < 0.05 is considered statistically significant). Bars represent mean ± SE (n = 3).