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. 2021 Jul 13;12(18):5454–5463. doi: 10.7150/jca.55727

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Silencing of CLDN6 decreased the migration and invasion capacity in hHCC cells HepG2 and Hep3B. A, representative light microscope images of wound-healing assays for HepG2 and Hep3B cells after CLDN6 silencing to evaluate their migration rate into the cell-free area (bar, 200 μm).B, Transwell assay (without Matrigel), cells that invaded through the Matrigel were stained with Giemsa and counted (bar, 50 μm) C, Matrigel invasion assay (bar, 50 μm). All results are presented as the average of cells counted in 10 fields per condition. (*, p < 0.05; **, p < 0.01).