FIG. 3.

Dietary iron deficiency prevented development of steatosis. PCBP1^fl/fl and PCBP1^Δhep mice were fed diets containing 5, 50, or 1,000 ppm of iron for 16–18 days. (A) Decreased steatohepatitis in iron-deficient PCBP1^Δhep livers by H&E staining of liver sections (left) and plasma ALT in mice fed an iron-deficient (5 ppm) diet. (B) Iron deficiency decreased accumulation of liver acylglycerides in PCBP1^Δhep mice. (C) Decreased expression of fatty acid biosynthetic genes in iron-deficient livers. For clarity, data for the 50-ppm iron diet are reproduced from Fig. 1. Data represent mean ± SD. Abbreviations: H&E, hematoxylin and eosin; ns, not significant; TAG, triacylglycerol.