FIGURE 3.

AdipoAI decreases inflammation in DIO mice (n = 6). DIO mice were orally gavaged with APR (50 mg kg⁻¹) or AdipoAI (25 mg kg⁻¹) for 14 days, and control animals (WT or DIO mice) were administered with equivalent volumes of DMSO. mRNA expression levels of proinflammatory cytokines in (a) WAT, (b) spleen and (c) bone marrow were measured by qRT-PCR and normalized to the level of β-actin mRNA. Data are expressed as mean ± SEM. (d) Serum levels of IL-6 and IL-1β were measured by ELISA. Data are expressed as mean ± SEM. Representative images of (e) H&E and (f) IHC of F4/80 staining of spleen tissue in DIO mice (n = 6). Red arrow indicates multinucleated giant cells (megakaryocytes). One-way ANOVA test for multiple group comparisons, * vs. WT vehicle group, # vs. DIO-vehicle group. */#P < 0.05, significant differences between each indicated group. ns, not significant