Fig. 1. MPP+ treatment affected the expression of UPRmt-related components.
SH-SY5Y cells were treated with 2 mM MPP+ for 2, 4, 8, 16, and 24 h. (A) Total cell lysates were harvested after MPP+ treatment and subjected to Western blot analyses with the indicated antibodies. Histograms show the relative density of (B) ClpP, (C) UBL5, (D) ClpX, and (E) LONP protein levels relative to total protein levels. The data are expressed as the mean ± SEM of three independent experiments. Data are compared with one-way ANOVA with Tukey’s post hoc test.