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. Author manuscript; available in PMC: 2022 Sep 10.
Published in final edited form as: Biochem Biophys Res Commun. 2021 Jun 30;569:17–22. doi: 10.1016/j.bbrc.2021.06.079

Fig. 2. MPP+ induced activation of the UPRmt at early time points.

Fig. 2.

(A) mRNA levels of ClpP, HSP60, and UBL5 were determined by real-time quantitative PCR in SH-SY5Y cells after treatment with 2 mM MPP+ for 2 and 8 h. (B) Total protein lysates were harvested from SH-SY5Y cells after treatment with 2 mM MPP+ for 2, 4, 8, 16, and 24 h and subjected to Western blot analyses with the indicated antibodies. The histogram shows the relative density of ATF5. The data are expressed as the mean ± SEM of three independent experiments. Data are compared with one-way ANOVA with Tukey’s post hoc test.