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. Author manuscript; available in PMC: 2022 Sep 10.
Published in final edited form as: Biochem Biophys Res Commun. 2021 Jun 30;569:17–22. doi: 10.1016/j.bbrc.2021.06.079

Fig. 4. Overexpression of ATF5 reduced MPP+-induced cellular stress.

Fig. 4.

SH-SY5Y cells were treated with 2 mM MPP+ after the overexpression of ATF5. (A) Total protein lysates were harvested after MPP+ treatment and subjected to Western blot analyses with the indicated antibodies. Histograms show the relative density of (B) ClpP, (C) ClpX, and (D) LONP protein levels. (E) Cells were stained with MitoSOX™ dye to assess mitochondrial superoxide production after 24 h treatment with MPP+. The histogram shows the relative fluorescence density of MitoSOX. (F) Cell viability was examined by the MTT assay after 24 h treatment with MPP+. The histogram shows relative cell survival. The data are expressed as the mean ± SEM of three independent experiments. Data are compared with one-way ANOVA with Tukey’s post hoc test.