FIGURE 4.

HMGA2 overexpression reversed hsa_circ_0008274 upregulated‐ or miR‐578 mimics‐mediated antitumor function. (a) The binding sequences between miR‐578 and HMGA2‐3′UTR were shown. The red sequences represent its mutant. (b),(c) The luciferase activity of HMGA2‐wt and HMGA2‐mut in A549 and SPC‐A‐1 cells was detected by dual‐luciferase reporter assay after transfection of miR‐578 or miR‐NC. (d) HMGA2 expression was determined by Western blot with or without miR‐578 mimics. (e),(f) Cell invasion was evaluated by the Transwell invasion. (g) The HMGA2 protein expression was measured after overexpressed circRNA cotransfected with or without miR‐578 mimics. (h),(i) Cell invasion was evaluated by the Transwell invasion. (j) The schematic diagram revealed that the downregulated hsa_circ_0008274 released miR‐578 and silences HMGA2 expression, which, in turn, affected the proliferation and invasion of LUAD