FIGURE 2.

SETD1A silencing leads to a proliferative defect in the NSCLC cell line. (a) Stable knockdown of SETD1A in human lung adenocarcinoma cells was generated by transfection of lentiviral‐encoded shRNA into the A549 cell line. Western blotting of A549 treated with different shRNA was performed and the quantification results are listed in the right panel (n = 4). (b) Stable knockdown of SETD1A in the H1299 NSCLC cell line. The efficiency of knockdown tested with Western blots and quantification results are listed in the right panel (n = 4). (c),(d) Cell number of A549 (c) or H1299 (d) with shRNA‐SETD1A (shSETD1A‐1/2) and shRNA‐scrambled (control) were recorded at indicated time points (n = 5). (e),(f) Western blots of cell cycle‐related proteins were tested and the quantification results are listed in the right panel (n = 4). Control, A549/ H1299 with lentivirus packaged shRNA for the scrambled sequence. shSETD1A‐1 or 2, A549/ H1299 with lentivirus packaged shRNA for SETD1A mRNA at different binding sites. Statistical tests were analyzed by using paired t‐test after log transformation. n means independent biological sample