FIGURE 5.

SETD1A knockdown in NSCLC cells decreases the expression of several critical oncogenes in lung cancer. (a),(b) Immunofluorescence of A549 cells (shSETD1A‐1 or control) with antibody to SETD1A or H3K4me3. The scale bar was 50 μm. (c)–(h) ChIP assay to detect location of H3K4me3 methylation in genomics. (i) Western blot for critical oncogenes with H3K4me3 methylation at the transcriptional start site. (j) Relative protein levels of oncogenic proteins in I. ACTB was used as the internal control (n = 4). P1 = 0.0193, P2 = 0.0385, P3 = 0.003, P4 = 0.0238, P5 = 0.0212, P6 = 0.0317, P7 = 0.0047, P8 = 0.0358, P9 = 0.0079, P10 = 0.0799, P11 = 0.0191, P12 = 0.0183. Statistical tests were analyzed by using paired t‐test after log transformation. n means independent biological sample