Figure 4.

CHD1 KO cells show more viable suspended cells than either RWPE-1 or NT2. (a) Plots depicting percent viability of cells growing in either an adherent (left) or suspended (right) fashion. In all groups, all cell lines showed similar viability. Percent viability of adherent cells was higher than that of suspended cells. Plots represent data from biological triplicates. Error bars represent standard deviation. (b) Plots demonstrating the number of viable cells from each of the five cell lines growing in either an adherent (left) or suspended (right) fashion on coated plates. Viable adherent cell numbers were not significantly different among the five lines on standard, laminin or fibronectin coated places. More Cr16 and Cr21 cells adhered on collagen I coated plates than RWPE-1, NT2, or Cr2 (^**p < 0.01). In addition, more Cr16 and Cr21 cells grew in suspension compared to RWPE-1, NT2, and Cr2 on all coated plates tested (^**p < 0.01). Error bars represent SD. (c) Plot depicting percent of viable suspended cells in total viable cells on different plates. Cr2, Cr16, and Cr21 had the most viable suspended cells on fibronectin and laminin coated plates. Error bars represent SD

CHDI, chromodomain helicase DNA binding protein 1; KO, knockout; NT2, non-target cells; SD, standard deviation.