Figure 6.

Generation of spheroids/acini in matrigel cultures. (a) Compact and irregular spheroids generated from RWPE-1, NT2, Cr2, Cr16, and Cr21 cell lines after being mixed with matrigel and grown for 10 days. (b) Plot depicting the number of compact and irregular spheroids from the five cell lines. All lines produced more irregular than compact spheroids. CHD1 KO lines generated more irregular spheroids than parental cells (^*p < 0.05, ^**p < 0.01). Data is from biological triplicates, and error bars represent SD. (c) Plot showing the number of cells that need to be plated to form an acinus from each cell line. RWPE-1 requires the largest number of cells, while Cr21 requires the fewest. Error bars represent SD.

CHD1, chromodomain helicase DNA binding protein 1; KO, knockout; NT2, non-target cells; SD, standard deviation.