Figure 3.

Transcriptome analysis of 5-HT neurons

(A) Principle-component analysis plot of nine independent samples for 5-HT neurons (S, three samples), non-patterned neurons (N, three samples), and undifferentiated hESCs (E, three samples).

(B) Bar plot summarizes the comparison of the numbers of significant differentially expressed genes between N and E with S.

(C) Venn diagram presents the overlap between the significantly differentially expressed genes.

(D and E) Selected lists of differentially expressed genes comparing S versus N (D) or S versus E (E). Red bars depict the genes upregulated in S (positive fold change), and blue bars indicate genes downregulated in S (negative fold change). All genes that are shown had an adjusted p < 0.05.

(F and G) GeneAnalytics Tissues & Cells analysis comparing upregulated gene lists of S versus N (F) or S versus E (G) with a database of genes expressed in specific human tissues and organs. DE, differentially expressed; PCA, principal-component analysis. See also Figures S2 and S3.