Figure 2.

Differentiation of PMPs into microglia in brain organoids

(A and G) (A) Representatives and (G) quantification of PU.1⁺ and PU.1⁺/Ki67⁺ cells in day 35 brain organoids. Scale bars, 20 μm. Pooled data from two hiPSC lines and one hESC line. Three independent experiments (n = 3) were performed, and, for each experiment, four to six organoids from each hPSC line were used. Data are presented as mean ± SEM.

(B) Representatives of IBA1⁺/CD45⁺ cells in day 45 brain organoids. Scale bars, 20 μm.

(C and H) (C) Representatives and (H) quantification of IBA1⁺ cells in day 50 brain organoids. Scale bars, 50 or 10 μm as indicated. Pooled data from two hiPSC lines and one hESC line. Three independent experiments (n = 3) were performed, and for each experiment, four to six organoids from each hPSC line were used. Data are presented as mean ± SEM.

(D) Representatives of CD11b⁺/CD45⁺ cells in day 55 brain organoids. Scale bars, 50 or 20 μm as indicated.

(E) Representatives of IBA1⁺/PU.1⁺ cells in day 55 brain organoids. Scale bars, 50 or 20 μm as indicated.

(F) Representatives of TMEM119⁺ cells in day 55 brain organoids. Scale bars, 20 μm.

See also Figure S1.