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. 2021 Feb 23;45(4):41. doi: 10.3892/or.2021.7992

Figure 3.

Figure 3.

Transient knockdown of JUN or FOS suppresses NUPR1 induction by nickel (Ni). (A) JUN mRNA expression and knockdown efficiency in BEAS-2B (left) and 293 (right) cells. (B) BEAS-2B (left) or 293 (right) cells were transfected with JUN siRNA (JUN KD), and either unexposed or exposed to 0.5 mM Ni for 24 h. NUPR1 mRNA was then assessed. (C) FOS mRNA expression and knockdown efficiency in BEAS-2B (left) and 293 (right) cells. (D) BEAS-2B (left) or 293 (right) cells were transfected with FOS siRNA (FOS KD), and either unexposed or exposed to 0.5 mM Ni for 24 h. NUPR1 mRNA was then assessed. Knockdown efficiencies of JUN or FOS were determined by measuring JUN or FOS expression in control siRNA (ctrl) cells compared to JUN or FOS expression in knockdown cells. Total RNA was extracted from transfected BEAS-2B and 293 cells or non-transfected BEAS-2B or 293 cells exposed to Ni. NUPR1 mRNA levels were measured by RT-qPCR. Gene expression levels were normalized to actin, and are presented as fold change relative to the control group. All data shown are the mean ± SD from qPCRs performed in triplicate. *P<0.05 and **P<0.01. NUPR1, nuclear protein 1.