Evaluating DAPI-stained nuclei quality under a fluorescent microscope
(A) 20× magnification of DAPI-stained nuclei via epifluorescence (top) and bright field (bottom) microscopy. The scale bar indicates 20 μm.
(B) Intact nuclei at 40× magnification via epifluorescence (top) and bright field (bottom) microscopy.
(C) Nuclei with intermediate ruptured nuclear envelopes at 40× magnification via epifluorescence (top) and bright field (bottom) microscopy.
(D) Lysed nuclei at 40× magnification via epifluorescence (top) and bright field (bottom) microscopy.