FIGURE 4.

Quantification of origin and terminus copy numbers in strain Zm4 under anaerobic conditions. (A) Growth curves of Zm4 strain under anaerobic conditions. Average OD₆₀₀ values of three biological replicates and their standard deviations are shown. Two sets of experiments (three biological replicates each) were performed, because in the first set of experiments the results in stationary phase did not meet the quality criteria (see different symbols and different dashed lines). Arrows indicate time points of collecting samples for quantification of genomic copy numbers. (B) Origin and termini copy numbers in anaerobically growing Zm4. Samples at an early stationary phase were collected about 1 h after the culture reached a plateau of its growth curve. All time points at growth phase are three biological replicates, while two biological replicates at an early stationary phase and 5 biological replicates were made for a late stationary phase. Standard deviations (STD) are presented as error bars.