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. 2021 Jul 23;40(16):e107911. doi: 10.15252/embj.2021107911

Figure 4. The effects of TOR inhibition on S6K phosphorylation and rates of protein synthesis.

Figure 4

Kinetic analyses on the identified phosphosites for the S6 kinase homologues in fission yeast, and the changes in rates of protein synthesis in the S6 kinase deletion mutant relative to wild type cells upon TOR inhibition.
  1. Relative protein expression levels of the 3 S6 kinase homologues, Psk1, Sck1 and Sck2, plotted against time of Torin1 addition. Respective proteins are represented by the different coloured lines as detailed in the legend.
  2. Relative phosphorylation level of the T632 phosphosite identified on Sck1 in the 40‐min time course study. Phosphorylation levels of both wild type (green) and tor1∆ (yellow) are presented relative to the value for wild type cells at T = 0 min. Grey dashed line indicates 2‐fold threshold.
  3. Relative phosphorylation of the T16 phosphosite identified on Sck2 in the 85‐min time course study (background adjusted detailed in Materials and Methods). Grey dashed line indicates 2‐fold threshold.
  4. Changes in the rates of protein synthesis upon Torin1 treatment (5 µM) measured for wild type and the S6 kinase deletion mutant (S6K 3x∆) plotted over time. Non‐linear regression exponential decay lines were fitted using the curve‐fitting function on Prism9. Error bars represent the standard deviation (SD) of data aggregated from 3 independent experiments.