Figure 1. SAGA and USP22 inducibly bind to the promoter region of ER stress response genes.

HCT116 human colorectal adenocarcinoma cells were treated for 2 h with 100 nM Thapsigargin to induce ER stress. Cells were cross‐linked, harvested, and subjected to high‐throughput ChIP‐sequencing for SAGA members USP22, GCN5, and ATXN7L3.

• A
  Genome browser images of ChIP‐seq for SAGA proteins before and after induction of ER stress at ER stress response genes CHOP, ERP70, and GRP78. ATF4/Xbp1s binding sites are from publicly available datasets (GEO accessions GSE69304 and GSE49952).
• B
  Heat map of USP22, GCN5, and ATXN7L3 association at the TSS for 29 genes whose promoters undergo significant recruitment of USP22 following induction of ER stress.
• C
  Mean ChIP‐seq signal for USP22, GCN5, and ATXN7L3 surrounding gene promoters from (B). Nonparametric Mann–Whitney analysis of 7 bins (gray shade) surrounding maximal peak in Thaps‐treated conditions is reported.
• D–F
  Linear regression analysis of SAGA member recruitment to target gene promoters following induction of ER stress.