Figure 3.

Internalization of chitooligosaccharide hexamer (COS6) in macrophages. (A) Here, 0.05 mM fluorescein isothiocyanate (FITC)-COS6 is internalized into macrophages by incubating for 10 min at 4°C, then the cell membrane was stained with 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) and the cell nucleus was stained with 4′,6-diamidino-2-phenylindole (DAPI). (B) The internalization of FITC-COS6 at different times (2, 4, 6, 8, and 10 min). Cells (1 × 10⁶–1 × 10⁷) were incubated with 0.05 mM FITC-COS6 at 28°C and examined by a confocal microscope. All the experiments were repeated at least three times. Scale bar:5 μm.