Skip to main content

View full-text article in PMC

. 2021 Aug 10;6(3):200–208. doi: 10.1016/j.synbio.2021.08.001

Table 1.

Editing efficiency for assessed genes based on endogenous NHEJ.

Gene ID^a	Guide sequence of sgRNA^b	Editing efficiency by NHEJ^c
*LPD07156*	GTGAGTACGCGTCGAACCGCTGG	3/10
*LPD02538*	CCTCCTCTGGCCAGCGCTTCAGG	0/24
*LPD03951*	GAAGAGTTACTTCCTCGAACCGG	3/11
*LPD01620*	CCGCTTCCACCGTCTCCTGCCGG	0/16
*LPD01622*	CAGGATCGTCAGCGCATTGCAGG	0/16
*LPD01628*	ATCTGCAACGGTTCCCGGACGGG	0/16

^a

LPD01620 encodes a subunit of gramicidin synthase and LPD01628 encodes an ATP-dependent DNA ligase.

^b

The guide sequence was designed using a web-based tool [22].

^c

Number of edited colonies/number of examined transformants.