Table 3.
Gene editing using the CRISPR/Cas9-based ssDNA recombineering system.
| Gene | Guide sequence of sgRNA | Oligonucleotidea | Deletion length | Editing efficiency |
|---|---|---|---|---|
| LPD07156 | GTGAGTACGCGTCGAACCGCTGG | Oligo-07156 | 2 | 8/23 (35 %) |
| LPD02538 | CCTCCTCTGGCCAGCGCTTCAGG | Oligo-02538 | 2 | 5/16 (31 %) |
| LPD01620 | CCGCTTCCACCGTCTCCTGCCGG | Oligo-01620 | 2 | 5/23 (22 %) |
| LPD01628 | ATCTGCAACGGTTCCCGGACGGG | Oligo-01628 | 2 | 11/23 (48 %) |
| LPD03951 | GAAGAGTTACTTCCTCGAACCGG | Oligo-03951-1 | 2 | 15/20 (75 %) |
| Oligo-03951-2 | 20 | 23/31 (74 %) | ||
| Oligo-03951-3 | 100 | 5/20 (25 %) | ||
| Oligo-03951-4 | 500 | 5/20 (25 %) | ||
| Oligo-03951-5 | 1000 | 0/20 (0 %) | ||
| Oligo-03951-6 | Point mutation | 36/36 (100 %) |
a
Sequences of the single-stranded oligonucleotides are listed in Table S4.