TABLE 1.

Test drugs were applied to colon explants in Krebs solution either by the perfusion system¹ or by pipetting them directly into the chamber².

Drug	Abbreviation	Final concentration in Krebs	Incubation time (min)	Target and drug action
D-tubocurarine1	dTC	100 μM	10–15	nAChR antagonist
Hexamethonium1	Hex	100 μM	10–15	nAChR antagonist
SR165841	SR	40 μM	10–15	α3β4-nAChR antagonist
Ondansetron1	Ondansetron	20 μM	10–15	5-HT3R antagonist
LY2785841	LY278584	10 μM	20	5-HT3R antagonist
Methyllycaconitine1	MLA	50 nM	60	α7-nAChR antagonist
Tetrodotoxin1	TTX	1 μM	15	Na+ channel blocker
Lidocaine1	Lido	2 mM	15	Na+ channel inhibitor
ω-conotoxin-MVIIC2	ω-CTx	100–200 nM	60	P/Q and N-type Ca2+ channel blocker
Pituitary adenylate cyclase activating polypeptide2	PACAP	100 nM	5	PAC1R, VPACR agonist