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. 2021 Aug 2;12:710794. doi: 10.3389/fpls.2021.710794

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Copyright © 2021 Dangol, Nguyen, Singh, Chen, Wang, Lee, Hwang and Jwa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative real-time RT-PCR analysis of time-course expression of OsNADP-ME and OsRbohB in rice leaf sheaths infected with avirulent Magnaporthe oryzae 007. Leaf sheaths of the wild-type (WT) rice cultivar DJ, OsMEK2 knock-out (ΔOsmek2 #2) and OsMEK2-overexpressed (35:OsMEK2 #4) plants were sampled at different time points after inoculation, followed by total RNA extraction. Relative gene expression levels of OsNADP-ME (Os01g52500) and OsRbohB (Os01g25820) at each time point were calculated by normalizing with respect to the expression of the internal control 18S rRNA (XR_003238819.1) gene. Data represent the means ± SD from three independent experiments. Different letters above the bars indicate significantly different means (P < 0.05), as analyzed by Fisher’s protected LSD test.