Figure 2.

Effects of G-Re on LPS-induced ROS production in BV2 microglial cells. BV2 cells were treated with G-Re for 1 h and then incubated with LPS (1 µg/ml) for 30 min. DMSO (0.04%) was used as vehicle. After incubation, the cells were treated with CM-H₂DCFDA for an additional 30 min. The intracellular levels of ROS were then determined by (A and B) flow cytometry and (C) fluorescence microscopy. (A) A representative histogram of flow cytometry is presented. Scale bar, 50 µm. *P<0.05 vs. the group treated with LPS alone. G-Re, ginsenoside Re; LPS, lipopolysaccharide; ROS, reactive oxygen species.