Figure 2.
Apoptosis of neurons in the perihematoma following ICH. (A) Protein expression levels and (B) semi-quantification of caspase-3 and cleaved caspase-3. Immunofluorescence of (C) NeuN, (D) cleaved caspase-3 and (E) Hoechst staining in the perihematoma region of the sham group. (F) Merged staining of the sham group (n=6). Immunofluorescence of (G) NeuN, (H) cleaved caspase-3 and (I) Hoechst staining in the perihematoma region at 2 d after ICH. (J) Merged staining at 2 d post-ICH. Immunofluorescence of (K) NeuN, (L) cleaved caspase-3 and (M) Hoechst staining in the perihematoma region at 7 d post-ICH. (N) Merged staining at 7 d post-ICH. *P<0.05 vs. sham. ICH, intracerebral hemorrhage; d, day.